American Society for Clinical Pathology (ASCP) Histotechnologist Practice Test

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Study for the ASCP Histotechnologist Test. Review comprehensive questions and explanations. Ace your exam with confidence!

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What is the main function of hydrolases in enzyme reactions?

To add water to substrates
To remove water from substrates
To oxidize substrates
To reduce substrates

The correct answer is: To add water to substrates

The main function of hydrolases in enzyme reactions is to catalyze the breakdown of substrates through the addition of water, a process known as hydrolysis. Hydrolases specifically facilitate the cleavage of chemical bonds in molecules, which often involves the insertion of a water molecule to break down complex substrates into simpler products. This catalytic action is essential in various biological processes, including digestion, where hydrolases help decompose macromolecules such as proteins, lipids, and carbohydrates into their monomeric units. The other options relate to different types of enzymatic reactions. The removal of water from substrates reflects dehydration reactions (not hydrolases), while oxidation and reduction involve other classes of enzymes altogether, such as oxidoreductases. Thus, understanding the role of hydrolases is key in the context of enzyme reactions and their importance in metabolic pathways.